Creating Higher Titer Lentivirus with Caffeine
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چکیده
منابع مشابه
Brief Reports Creating Higher Titer Lentivirus with Caffeine
The use of lentiviral vectors extends from the laboratory, where they are used for basic studies in virology and as gene transfer vectors gene delivery, to the clinic, where clinical trials using these vectors for gene therapy are currently underway. Lentiviral vectors are useful for gene transfer because they have a large cloning capacity and a broad tropism. Although procedures for lentiviral...
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Chen Chu1,2, Aijie Xin1,3,4, Yuchuan Zhou1, and Yonglian Zhang1,3* Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China Graduate University of the Chinese Academy of Sciences, Shanghai 200031, China Shanghai Institute...
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Lentiviral technology has proven to be a powerful tool to express exogenous genes in dividing and non-dividing cells. Currently, most protocols for generating high-titer lentivirus require ultracentrifugation, which can be an instrumental barrier for routine operations in a laboratory. In this study, the effect of relative centrifugal force (RCF) on the concentration efficiency of the lentiviru...
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Lion lentivirus (LLV; also known as feline immunodeficiency virus of lion, Panthera leo [FIVPle]) is present in free-ranging and captive lion populations at a seroprevalence of up to 100%; however, clinical signs are rarely reported. LLV displays up to 25% interclade sequence diversity, suggesting that it has been in the lion population for some time and may be significantly host adapted. Three...
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Caffeine is a purine, l, 3, 7-trimethyl xanthine. Since it is consumed in great quantities by man, the question concerning whether it is a mutagen for man is of great significance. There is now good evidence that caffeine is mutagenic not only in bacteria (4, 5, 7, 9,18), fungi (6,41), and plants (11, 12), but equally mutagenic in Drosophila melanogaster [2, 16, 25, 29; negative reports, (40)],...
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ژورنال
عنوان ژورنال: Human Gene Therapy
سال: 2011
ISSN: 1043-0342,1557-7422
DOI: 10.1089/hum.2010.068